Gene regulation of rice storage protein glutelin.
نویسندگان
چکیده
منابع مشابه
Elements Responsible for Quantitative Regulation of Rice Seed Storage Protein Glutelin GlttA - 3 Gene the
The major seed storage protein of rice (Oryza sativa L.), glutelin, is encoded by a smal1 multigene family of about 10 copies per haploid genome. Eight genes have a)ready been sequenced at both the cDNA and genomic DNA levels (Takaiwa et al. 1987a, b, 1989, 1991a, c, Okita et al, 1989), All the glutelin genes are specifically expressed in the maturing seed (Okita et al. 1989, Takaiwa et al. 199...
متن کاملStructural Relationship among the Rice Glutelin Polypeptides.
When the glutelin protein fraction of rice (Oryza sativa L.) seeds was fractionated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, three size classes of proteins, 51 kilodaltons (kD), 34 to 37 kD, and 21 to 22 kD, as well as a contaminating prolamine polypeptide of 14 kD were detected. Antibodies were raised against these proteins and employed in studies to determine whether a pr...
متن کاملExpression pattern and activity of six glutelin gene promoters in transgenic rice*
The shortage of strong endosperm-specific expression promoters for driving the expression of recombinant protein genes in cereal endosperm is a major limitation in obtaining the required level and pattern of expression. Six promoters of seed storage glutelin genes (GluA-1, GluA-2, GluA-3, GluB-3, GluB-5, and GluC) were isolated from rice (Oryza sativa L.) genomic DNA by PCR. Their spatial and t...
متن کاملCharacterization of a new rice glutelin gene GluD-1 expressed in the starchy endosperm
A new glutelin gene, designated GluD-1, has been discovered by comparing the seed storage proteins from 48 japonica and indica rice cultivars on SDS-PAGE gels. Evidence that GluD-1 is a member of the glutelin family was provided by Western blots using anti-glutelin antiserum and by mapping the gene to the chromosomal glutelin gene cluster. The limited GluD-1 size polymorphism among the rice var...
متن کاملRegulation of storage protein gene expression in Arabidopsis.
The expression of seed storage proteins is under tight developmental regulation and represents a powerful model system to study the regulation of gene expression during plant development. In this study, we show that three homologous B3 type transcription factors regulate the model storage protein gene, At2S3, via two distinct mechanisms: FUSCA3 (FUS3) and LEAFY COTYLEDON2 (LEC2) activate the At...
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ژورنال
عنوان ژورنال: Kagaku To Seibutsu
سال: 1990
ISSN: 0453-073X,1883-6852
DOI: 10.1271/kagakutoseibutsu1962.28.448